- Title
- Comparative proteomics of the toxigenic diazotroph Raphidiopsis raciborskii (cyanobacteria) in response to iron
- Creator
- D'Agostino, Paul M.; Yeung, Anna C. Y.; Poljak, Anne; David Waite, Trevor; Neilan, Brett A.
- Relation
- ARC.LP0883561
- Relation
- Environmental Microbiology Vol. 23, Issue 1, p. 405-414
- Publisher Link
- http://dx.doi.org/10.1111/1462-2920.15328
- Publisher
- Wiley-Blackwell
- Resource Type
- journal article
- Date
- 2020
- Description
- Raphidiopsis raciborskii is an invasive bloom-forming cyanobacteria with the flexibility to utilize atmospheric and fixed nitrogen. Since nitrogen-fixation has a high requirement for iron as an ezyme cofactor, we hypothesize that iron availability would determine the success of the species under nitrogen-fixing conditions. This study compares the proteomic response of cylindrospermopsin-producing and non-toxic strains of R. racibroskii to reduced iron concentrations, under nitrogen-fixing conditions, to examine any strain-specific adaptations that might increase fitness under these conditions. We also compared their proteomic responses at exponential and stationary growth phases to capture the changes throughout the growth cycle. Overall, the toxic strain was more competitive under Fe-starved conditions during exponential phase, with upregulated growth and transport-related proteins. The non-toxic strain showed reduced protein expression across multiple primary metabolism pathways. We propose that the increased expression of porin proteins during the exponential growth phase enables toxic strains to persist under Fe-starved conditions with this ability providing a potential explanation for the increased fitness of cylindrospermoipsin-producing strains during unfavourable environmental conditions.
- Subject
- cyanobacteria; nitrogen-fixing conditions; growth cycle; toxic strain
- Identifier
- http://hdl.handle.net/1959.13/1430458
- Identifier
- uon:38846
- Identifier
- ISSN:1462-2912
- Rights
- This is the peer reviewed version of the following article: D'Agostino, Paul M.; Yeung, Anna C. Y.; Poljak, Anne; David Waite, Trevor; Neilan, Brett A. “Comparative proteomics of the toxigenic diazotroph Raphidiopsis raciborskii (cyanobacteria) in response to iron.” Environmental Microbiology Vol. 23, Issue 1, 405-414 (2020), which has been published in final form at http://dx.doi.org/10.1111/1462-2920.15328 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
- Language
- eng
- Full Text
- Reviewed
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